On Monday of this week, I got equipment for the DMC trip ready and packed. I also started to set up the website that will contain all the spectral data we have collected. I have already added most of the new data we collected on Tuesday to the site. I have pictures and names corresponding to the each spectrum. The names are specific as possible, some organisms I have tagged with genus and species, others just genus. Then I have just substrates (e.g. sand, shell, mud). All data on the site is referenced to the 99% reflectance standard. I applied the equitation I derived last week to all the Satalantic radiometer data. It’s looking pretty good so far, come check it out next week sometime. Hopefully next week I can work with Lisa Taylor to get both the float site and the reflectance data site up and running.


Ok, now to the data we collected on Tuesday. The Satalantic data matched up excellent with Mike’s notes! No trouble there. The DiveSpec data does not match up quite as well, the headings and Mike’s notes differ a couple times, but I have being using the headings entered by Emmanuel as the final decider in these situations. With the DiveSpec, six Didemnum spectra were taken,

I Hope these look familiar to you by now, it’s quite obvious when you are looking at Didemnum. Only one seems slightly different than the rest. Emmanuel told me that “D2” was not a prime specimen of Didemnum, however the outlier we see here is not “D2”, just so you know. Wayne and I also did some work in the touch tank with the DiveSpec, these were our results.

The “unknown tunicate” was just that. We took two pictures of it, but they came out blurry. Besides the unknown tunicate everything is significantly different from Didemnum. There is a lot of DiveSpec data, so I am not going to put all the graphs up. I will work on condensing and adding it all to the website next week. With the Satlantic radiometer we collected two Didemnum spectra.

The dashed lines are the original spectra; the solid line is after it had been referenced to the 99% reflectance standard. These look similar to our light data we collected last time we were at DMC. Here is another comparative plot but using the some of the Satlantic data. The numbers next to the name in legend are the 550/500 ratio.

 After I organized all the Didemnum spectra I calculated the 550/500 ratio again.


Dive                       Mean               Stand Dev.

1(DiveSpec)          2.5822              0.6986

2(HyperOCR)       2.2518              0.2441

3(DiveSpec)         1.7056               0.1788

4(HyperOCR)      1.5939               0.1774

Total                     2.1514              0.5034



Dive 1 also includes the Didemnum spectra Wayne and I took in the lab. The first time we went, both the means are above two and the second time we went, both are below two. It is possible that we see a difference between the two trips to DMC because conditions were different. Also perhaps we sampled old growth the first time and new growth on the second trip. Regardless, dive 1 is definitely an outlier. The mean is very high and so is the standard deviation. We may have to reevaluate that data, because some spectra have very low reflectance, perhaps the DiveSpec was not functioning correctly.

We also have a significant amount of fluorescence data. Emmanuel took four fluorescence readings of Didemnum.

There all seem to have a small bump around 550, it is rather small however. I suppose this is good news. Wayne and I also collected fluorescence data in the touch tanks. Here are four the six fluorescence readings we took.

Note that the scale is different for each plot. Just like Didemnum, the sea star shows a similar rise at 550, which is unfortunate. As you can see both macroalgae show a large spike around 680.

I have most of the data we collected organized and named. As you know, next week is my last week of work. I will compile all the data and some of the m files I wrote and pass them on to Wayne. I will try to organize everything so he will know what is what. Hopefully i can wrap everything up next week, I will try my best to get everything done, websites finished and launch, stress data added to my current MVCO data, and all spec data organized and in Wayne's hands. See you next week.